ABSTRACT
Abstract Natural products are considered an important source of the therapeutic arsenal currently available. Among these alternatives are the seeds of Ambrosia peruviana (altamisa), whose extract has shown an anti-inflammatory effect. The main objective of this work was to perform a preformulation study of Ambrosia peruviana seeds ethanolic extract, where the main factors that affect the physical, chemical, and pharmacological stability of the extract were evaluated, as well as a compatibility study by differential scanning calorimetry (DSC) analysis against different excipients. A dry extract was obtained by rotary evaporation of the seeds macerated with 96% ethanol. The anti-inflammatory activity was determined by measuring its effect on NO production in RAW 264.7 macrophages, stimulated with LPS. The results showed that the dry extract maintained its stability over time when stored at a temperature of 4 and 25ºC, demonstrating its biological activity, the content of phenolic compounds, and its physicochemical parameters remain practically invariable. However, when exposed to high temperatures (60 ºC) it was affected. The thermal analysis revelated that the behavior of most of the selected excipients and the dry extract was maintained, which indicates that it did not present incompatibilities, therefore they can be candidates for formulating a microemulsion.
Subject(s)
Seeds/metabolism , Asteraceae/classification , Ambrosia/adverse effects , Biological Products , Calorimetry, Differential Scanning/methods , Excipients/administration & dosageABSTRACT
Introducción: Los trastornos alimentarios en menores de 5 años pueden causar alteraciones en el desarrollo y crecimiento. Pueden asociarse con trastornos de la funcionalidad familiar. Objetivo: Describir los trastornos de alimentación de tipo no orgánico en menores de 5 años y su asociación con la funcionalidad familiar en un hospital de segundo nivel en Puebla. Métodos: Estudio descriptivo y transversal, en el que se incluyeron niños menores de 5 años con trastornos alimentarios no orgánicos. A los padres que previamente firmaron el consentimiento informado se les aplicó la herramienta IMFED para estudio de los trastornos alimenticios, y FACES III para describir la funcionalidad familiar. Se utilizó estadística descriptiva y coeficiente de Spearman. Resultados: Se evaluaron 105 pacientes de los cuales 57 fueron hombres y 48 mujeres. La edad media fue 30,42 mínimo 2, máximo 60, ± 16,68 meses. El grupo etario predominante fue de 12-23 meses. 45,71% de los padres reportaron adaptabilidad familiar caótica y 39,04% cohesión relacionada. El trastorno de alimentación más frecuente fue aversiones sensoriales al alimento. Más del 60% presentaron dos o más trastornos de alimentación. La correlación entre adaptabilidad familiar y trastornos de alimentación fue 0,248 (p=0.011), y entre cohesión familiar y trastornos de alimentación 0,87 (p=0,38). Conclusión: Los trastornos de alimentación más frecuentes fueron aversión sensorial al alimento y anorexia infantil. Se correlacionan levemente con adaptabilidad familiar. Son más frecuentes en familias con adaptabilidad caótica. Los menores de 5 años con dos o más trastornos alimentarios son los más frecuentes.
Introduction: Eating disorders under 5 years old may cause development and grow problems. Disorders in family functionality can be associated. Objective: To describe non-organic eating disorders in children under 5 years of age and their association with family functionality in a second-level hospital in Puebla. Methods: It was a descriptive, cross-sectional study. Children under 5 years old with non-organic eating disorders were included. A questionnaire to study eating disorders (IMFED) and other one to describe family functionality (FACES III) were applied to their parents who previously signed informed consent. Descriptive statistics and Spearman test were used. Results: 105 patients were evaluated, 57 men and 48 women, medium age 3.042, minimum 2, maximum 60, ± 16.68 months old. Children 12-23 months old were more frequent. Parents reported chaotic family adaptability in 45.71%, and 39.04% related cohesion. The most frequent eating disorder was sensorial aversions to food. More than 60% had two or more eating disorders. Family adaptability and eating disorders were correlated in 0.248(p=0.011), and family cohesion and eating disorders 0.87 (p=0.38). Conclusion: Most frequent eating disorders were sensorial aversion to food and infant anorexia. They softly correlate with family adaptability. Children under 5 years old with 2 or more eating disorders are more frequent.